Analysis of Quinoa Saponin Extract and Blood Constituents Based on UPLC-Q-Exactive-MS/MS

Weihong WANG; Juli HU; Dingtao WU; Shijie WANG; Hong JIANG; Liang ZOU; Yichen HU

doi:10.13386/j.issn1002-0306.2022050100

Shipin gongye ke-ji (May 2023)

Analysis of Quinoa Saponin Extract and Blood Constituents Based on UPLC-Q-Exactive-MS/MS

Weihong WANG,
Juli HU,
Dingtao WU,
Shijie WANG,
Hong JIANG,
Liang ZOU,
Yichen HU

Affiliations

Weihong WANG: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Juli HU: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Dingtao WU: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Shijie WANG: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Hong JIANG: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Liang ZOU: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Yichen HU: Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China

DOI: https://doi.org/10.13386/j.issn1002-0306.2022050100
Journal volume & issue: Vol. 44, no. 9
pp. 296 – 308

Abstract

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The main chemical components of saponins extracted from quinoa and the components in the blood of rat after oral administration were analyzed and identified by ultra-performance liquid chromatography coupled with Q-Exactive mass spectrometry (UPLC-Q-Exactive-MS/MS). Hypersil Gold VANQUISH C18 column (2.1 mm×100 mm，1.8 μm) was used. The mobile phase was formic acid water-formic acid acetonitrile gradient elution, the column temperature was 30 ℃, the analysis time was 35 min, the flow rate was 0.3 mL·min−1. Electrospray ion source (ESI), positive and negative ion sources and Full ms/dd-ms2 mode were used for detection. The results showed that the recovery rate, matrix effect, precision and stability of the method met the requirements of biological samples. A total of 15 saponins were identified in the saponin extract of quinoa, which were divided into three oleanolic acid saponin types, five hederagenin saponin types, six phytolaccagenic acid saponin types and one serjanic acid saponin type according to the configuration of aglycones. Male Sprague Dawley (SD) rats were selected as the experimental object, after intragastric administration of quinoa saponin extract at 175.5 mg·kg−1, at 0, 0.5, 1, 2 and 4 hours after administration, blood was taken from the orbit, the plasma of rats took buspirone hydrochloride as the internal standard, precipitated protein with methanol, centrifuged, filtered by microporous membrane, and then injected for analysis. The results showed that six prototype saponins and trace hydrolyzed hederagenin and serjanic acid glycosides were detected in the blood components of rat plasma. Through the analysis of the components of the extract and the components entering the blood, 15 saponins in quinoa saponins were identified and their cleavage laws were found, and the changes in the relative contents of the components into the blood of rat plasma were found. The chemical composition and metabolic characteristics of triterpenoid saponins in quinoa were preliminaries elucidated, which provided a theoretical basis for further research and application of quinoa.

Published in Shipin gongye ke-ji

ISSN: 1002-0306 (Print)
Publisher: The editorial department of Science and Technology of Food Industry
Country of publisher: China
LCC subjects: Technology: Chemical technology: Food processing and manufacture
Website: http://www.spgykj.com/indexen.htm

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