Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis

Jingfeng Yang; Meng Yi; Jinfeng Pan; Jun Zhao; Liming Sun; Xinping Lin; Yuegang Cao; Lu Huang; Beiwei Zhu; Chenxu Yu

Journal of Functional Foods (Apr 2015)

Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis

Jingfeng Yang,
Meng Yi,
Jinfeng Pan,
Jun Zhao,
Liming Sun,
Xinping Lin,
Yuegang Cao,
Lu Huang,
Beiwei Zhu,
Chenxu Yu

Affiliations

Jingfeng Yang: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China; Corresponding author. School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggongyuan, Ganjingzi district, Dalian 116034, China. Tel.: +86 411 86323262; fax: +86 411 86323262.
Meng Yi: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Jinfeng Pan: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Jun Zhao: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Liming Sun: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Xinping Lin: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Yuegang Cao: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Lu Huang: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Beiwei Zhu: School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
Chenxu Yu: Department of Agricultural and Biosystems Engineering, Iowa State University, IA 50011, USA

Journal volume & issue: Vol. 14
pp. 519 – 528

Abstract

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A new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin (Strongylocentrotus intermedius), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the SUP is homogenous with an average molecular weight of 4.4 kDa, and it is mainly composed of mannose with a small number of glucose and N-acetylglucosamine, with a backbone of alternating 1, 4-D-Man and 1, 3-D-Man residues. The SUP was shown to synergistically enhance BMP-2 induced osteoblastic differentiation in C2C12 myoblast cells, evidenced by a significant increase in the activity of alkaline phosphatase (ALP), when the concentration of the SUP was higher than 10 µg/mL. The elevated ALP activity was also confirmed by histochemical staining of C2C12 myoblast cells. These results suggest that the SUP can promote bone regeneration and may serve as a functional food supplement for bone health.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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