Polysaccharides isolated from Hibiscus sabdariffa L. mitigate intestinal radiation injury through relieving mucosal inflammation and reshaping gut microbiota in mice

Abstract

Hibiscus sabdariffa L. (H. sabdariffa) shows considerable promise in alleviating oxidative stress injury through its rich content of phytochemicals, especially polysaccharides, yet its efficacy in treating intestinal radiation injury remains unclear. Here, we purified one water-soluble polysaccharides from H. sabdariffa (HSP1), and subsequently explored its role in relieving intestinal radiation injury via a mouse model of abdominal irradiation. The results demonstrated that HSP1 intervention effectively improved gut barrier function by restoring mucus secretion and upregulating tight junction proteins. Additionally, HSP1 mitigated oxidative stress through Nrf2/HO-1 pathway and reduced the inflammatory response through restraining the activation of pattern recognition receptors. Moreover, HSP1 modulated the balance of gut microbiota by suppressing pathogenic bacteria, while promoting the growth of beneficial symbiotic bacteria such as Lactobacillus, Ligilactobacillus, and Lachnospiraceae_NK4A136. Altogether, this study highlights the protective potential of HSP1 against intestinal radiation injury, providing primary data for further applications of H. sabdariffa polysaccharides as radioprotectors.

Keywords

• Gut microbiota
• Hibiscus sabdariffa L.
• Intestinal radiation injury
• Mucosal inflammation
• Oxidative stress
• Polysaccharides