npj Vaccines (Oct 2021)
A SARS-CoV-2 spike ferritin nanoparticle vaccine protects hamsters against Alpha and Beta virus variant challenge
- Kathryn McGuckin Wuertz,
- Erica K. Barkei,
- Wei-Hung Chen,
- Elizabeth J. Martinez,
- Ines Lakhal-Naouar,
- Linda L. Jagodzinski,
- Dominic Paquin-Proulx,
- Gregory D. Gromowski,
- Isabella Swafford,
- Akshaya Ganesh,
- Ming Dong,
- Xiankun Zeng,
- Paul V. Thomas,
- Rajeshwer S. Sankhala,
- Agnes Hajduczki,
- Caroline E. Peterson,
- Caitlin Kuklis,
- Sandrine Soman,
- Lindsay Wieczorek,
- Michelle Zemil,
- Alexander Anderson,
- Janice Darden,
- Heather Hernandez,
- Hannah Grove,
- Vincent Dussupt,
- Holly Hack,
- Rafael de la Barrera,
- Stasya Zarling,
- James F. Wood,
- Jeffrey W. Froude,
- Matthew Gagne,
- Amy R. Henry,
- Elham Bayat Mokhtari,
- Prakriti Mudvari,
- Shelly J. Krebs,
- Andrew S. Pekosz,
- Jeffrey R. Currier,
- Swagata Kar,
- Maciel Porto,
- Adrienne Winn,
- Kamil Radzyminski,
- Mark G. Lewis,
- Sandhya Vasan,
- Mehul Suthar,
- Victoria R. Polonis,
- Gary R. Matyas,
- Eli A. Boritz,
- Daniel C. Douek,
- Robert A. Seder,
- Sharon P. Daye,
- Mangala Rao,
- Sheila A. Peel,
- M. Gordon Joyce,
- Diane L. Bolton,
- Nelson L. Michael,
- Kayvon Modjarrad
Affiliations
- Kathryn McGuckin Wuertz
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Erica K. Barkei
- Veterinary Pathology Division, Walter Reed Army Institute of Research
- Wei-Hung Chen
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Elizabeth J. Martinez
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Ines Lakhal-Naouar
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Linda L. Jagodzinski
- Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Dominic Paquin-Proulx
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Gregory D. Gromowski
- Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Isabella Swafford
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Akshaya Ganesh
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Ming Dong
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Xiankun Zeng
- Pathology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick
- Paul V. Thomas
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Rajeshwer S. Sankhala
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Agnes Hajduczki
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Caroline E. Peterson
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Caitlin Kuklis
- Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Sandrine Soman
- Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Lindsay Wieczorek
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Michelle Zemil
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Alexander Anderson
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Janice Darden
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Heather Hernandez
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Hannah Grove
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Vincent Dussupt
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Holly Hack
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Rafael de la Barrera
- Pilot Bioproduction Facility, Walter Reed Army Institute of Research
- Stasya Zarling
- Pilot Bioproduction Facility, Walter Reed Army Institute of Research
- James F. Wood
- Pilot Bioproduction Facility, Walter Reed Army Institute of Research
- Jeffrey W. Froude
- Pilot Bioproduction Facility, Walter Reed Army Institute of Research
- Matthew Gagne
- Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Amy R. Henry
- Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Elham Bayat Mokhtari
- Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Prakriti Mudvari
- Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Shelly J. Krebs
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Andrew S. Pekosz
- W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health
- Jeffrey R. Currier
- Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Swagata Kar
- BioQual, Inc.
- Maciel Porto
- BioQual, Inc.
- Adrienne Winn
- BioQual, Inc.
- Kamil Radzyminski
- BioQual, Inc.
- Mark G. Lewis
- BioQual, Inc.
- Sandhya Vasan
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Mehul Suthar
- Emory Vaccine Center, Department of Pediatrics, Emory School of Medicine
- Victoria R. Polonis
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Gary R. Matyas
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Eli A. Boritz
- Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Daniel C. Douek
- Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Robert A. Seder
- Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Sharon P. Daye
- One Health Branch, Walter Reed Army Institute of Research
- Mangala Rao
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Sheila A. Peel
- Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- M. Gordon Joyce
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Diane L. Bolton
- U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Nelson L. Michael
- Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- Kayvon Modjarrad
- Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research
- DOI
- https://doi.org/10.1038/s41541-021-00392-7
- Journal volume & issue
-
Vol. 6,
no. 1
pp. 1 – 11
Abstract
Abstract The emergence of SARS-CoV-2 variants of concern (VOC) requires adequate coverage of vaccine protection. We evaluated whether a SARS-CoV-2 spike ferritin nanoparticle vaccine (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against the Alpha (B.1.1.7), and Beta (B.1.351) VOCs in Syrian golden hamsters. SpFN-ALFQ was administered as either single or double-vaccination (0 and 4 week) regimens, using a high (10 μg) or low (0.2 μg) dose. Animals were intranasally challenged at week 11. Binding antibody responses were comparable between high- and low-dose groups. Neutralizing antibody titers were equivalent against WA1, B.1.1.7, and B.1.351 variants following two high dose vaccinations. Dose-dependent SpFN-ALFQ vaccination protected against SARS-CoV-2-induced disease and viral replication following intranasal B.1.1.7 or B.1.351 challenge, as evidenced by reduced weight loss, lung pathology, and lung and nasal turbinate viral burden. These data support the development of SpFN-ALFQ as a broadly protective, next-generation SARS-CoV-2 vaccine.
