Immunoprofiles associated with controlled human malaria infection and naturally acquired immunity identify a shared IgA pre-erythrocytic immunoproteome
Andrea A. Berry,
Joshua M. Obiero,
Mark A. Travassos,
Amed Ouattara,
Drissa Coulibaly,
Matthew Adams,
Rafael Ramiro de Assis,
Aarti Jain,
Omid Taghavian,
Andrew Sy,
Rie Nakajima,
Algis Jasinskas,
Matthew B. Laurens,
Shannon Takala-Harrison,
Bourema Kouriba,
Abdoulaye K. Kone,
Ogobara K. Doumbo,
B. Kim Lee Sim,
Stephen L. Hoffman,
Christopher V. Plowe,
Mahamadou A. Thera,
Philip L. Felgner,
Kirsten E. Lyke
Affiliations
Andrea A. Berry
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Joshua M. Obiero
Department of Physiology and Biophysics, School of Medicine, University of California
Mark A. Travassos
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Amed Ouattara
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Drissa Coulibaly
Malaria Research and Training Center, University of Sciences, Techniques, and Technologies of Bamako
Matthew Adams
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Rafael Ramiro de Assis
Department of Physiology and Biophysics, School of Medicine, University of California
Aarti Jain
Department of Physiology and Biophysics, School of Medicine, University of California
Omid Taghavian
Department of Physiology and Biophysics, School of Medicine, University of California
Andrew Sy
Department of Physiology and Biophysics, School of Medicine, University of California
Rie Nakajima
Department of Physiology and Biophysics, School of Medicine, University of California
Algis Jasinskas
Department of Physiology and Biophysics, School of Medicine, University of California
Matthew B. Laurens
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Shannon Takala-Harrison
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Bourema Kouriba
Malaria Research and Training Center, University of Sciences, Techniques, and Technologies of Bamako
Abdoulaye K. Kone
Malaria Research and Training Center, University of Sciences, Techniques, and Technologies of Bamako
Ogobara K. Doumbo
Malaria Research and Training Center, University of Sciences, Techniques, and Technologies of Bamako
B. Kim Lee Sim
Sanaria Inc.
Stephen L. Hoffman
Sanaria Inc.
Christopher V. Plowe
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Mahamadou A. Thera
Malaria Research and Training Center, University of Sciences, Techniques, and Technologies of Bamako
Philip L. Felgner
Department of Physiology and Biophysics, School of Medicine, University of California
Kirsten E. Lyke
Center for Vaccine Development and Global Health, University of Maryland School of Medicine
Abstract Knowledge of the Plasmodium falciparum antigens that comprise the human liver stage immunoproteome is important for pre-erythrocytic vaccine development, but, compared with the erythrocytic stage immunoproteome, more challenging to classify. Previous studies of P. falciparum antibody responses report IgG and rarely IgA responses. We assessed IgG and IgA antibody responses in adult sera collected during two controlled human malaria infection (CHMI) studies in malaria-naïve volunteers and in 1- to 6-year-old malaria-exposed Malian children on a 251 P. falciparum antigen protein microarray. IgG profiles in the two CHMI groups were equivalent and differed from Malian children. IgA profiles were robust in the CHMI groups and a subset of Malian children. We describe immunoproteome differences in naïve vs. exposed individuals and report pre-erythrocytic proteins recognized by the immune system. IgA responses detected in this study expand the list of pre-erythrocytic antigens for further characterization as potential vaccine candidates.
