Coenzyme Q0, a quinone derivative from Antrodia camphorata, inhibits epithelial-mesenchymal transition by activating the Nrf2 signaling pathway in TGF-β-stimulated adult retinal pigment epithelial cells to improve age-related macular degeneration and proliferative vitreoretinopathy

Hsin-Ling Yang; Hung-Rong Yen; I-Chen Chang; Chithravel Vadivalagan; Siang-Jyun Chen; Chuan-Chen Lee; Wei-Chen Jane; Jhih-Hsuan Hseu; You-Cheng Hseu

Journal of Functional Foods (Jan 2024)

Coenzyme Q0, a quinone derivative from Antrodia camphorata, inhibits epithelial-mesenchymal transition by activating the Nrf2 signaling pathway in TGF-β-stimulated adult retinal pigment epithelial cells to improve age-related macular degeneration and proliferative vitreoretinopathy

Hsin-Ling Yang,
Hung-Rong Yen,
I-Chen Chang,
Chithravel Vadivalagan,
Siang-Jyun Chen,
Chuan-Chen Lee,
Wei-Chen Jane,
Jhih-Hsuan Hseu,
You-Cheng Hseu

Affiliations

Hsin-Ling Yang: Institute of Nutrition, College of Health Care, China Medical University, Taichung 40402, Taiwan
Hung-Rong Yen: Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan; Research Center of Chinese Herbal Medicine, China Medical University, Taichung 40402, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan; School of Chinese Medicine, China Medical University, Taichung40402, Taiwan
I-Chen Chang: Institute of Nutrition, College of Health Care, China Medical University, Taichung 40402, Taiwan
Chithravel Vadivalagan: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States
Siang-Jyun Chen: Institute of Nutrition, College of Health Care, China Medical University, Taichung 40402, Taiwan
Chuan-Chen Lee: Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan
Wei-Chen Jane: Institute of Translational Medicine and New Drug Development, China Medical University, Taichung 40402, Taiwan
Jhih-Hsuan Hseu: Department of Dermatology, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan; Corresponding authors at: Department of Cosmeceutics, College of Pharmacy, China Medical University, Shui-Nan Campus, No. 100, Sec. 1, Jingmao Road, Beitun District, Taichung City, Taiwan, Department of Dermatology, Kaohsiung Chang Gung Memorial Hospital, No. 123, Dapi Road, Niaosong District, Kaohsiung 83301, Taiwan.
You-Cheng Hseu: Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan; Research Center of Chinese Herbal Medicine, China Medical University, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan; Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung 40402, Taiwan; Corresponding authors at: Department of Cosmeceutics, College of Pharmacy, China Medical University, Shui-Nan Campus, No. 100, Sec. 1, Jingmao Road, Beitun District, Taichung City, Taiwan, Department of Dermatology, Kaohsiung Chang Gung Memorial Hospital, No. 123, Dapi Road, Niaosong District, Kaohsiung 83301, Taiwan.

Journal volume & issue: Vol. 112
p. 105978

Abstract

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We investigated the anti-EMT and antifibrotic effects of coenzyme Q0 (CoQ0), a quinone derivative from Antrodia camphorata, in TGF-β-stimulated adult retinal pigment epithelial 19 (ARPE-19) cells. Results showed that CoQ0 treatment reversed TGF-β-stimulated morphological changes from an epithelial to a fibroblastic phenotype in ARPE-19 cells. CoQ0 exhibited anti-EMT effects by impeding TGF-β-stimulated migration and invasion in ARPE-19 cells. Notably, CoQ0 triggered the epithelial marker E-cadherin and suppressed the mesenchymal marker N-cadherin expression in nonstimulated or TGF-β-stimulated ARPE-19 cells. Moreover, CoQ0 attenuated EMT and fibrotic Vimentin, MMP-9/-2, Slug, α-SMA, and VEGF expression in nonstimulated or TGF-β-stimulated ARPE-19 cells. Interestingly, CoQ0 inhibited TGF-β-induced intracellular ROS production by activating Nrf2 nuclear translocation and upregulating the HO-1, γ-GCLC, and NQO-1 enzymes in ARPE-19 cells. Moreover, Nrf2 silencing reversed TGF-β-induced ROS-mediated anti-EMT (E-cadherin/N-cadherin/Slug) and antifibrotic (α-SMA) effects in CoQ0-treated ARPE-19 cells. Therefore, CoQ0 could be utilized to treat age-related macular degeneration and proliferative vitreoretinopathy.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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