Exopolysaccharides from <i>Lactiplantibacillus plantarum</i> C7 Exhibited Antibacterial, Antioxidant, Anti-Enzymatic, and Prebiotic Activities
Taroub Bouzaiene,
Mariem Mohamedhen Vall,
Manel Ziadi,
Ines Ben Rejeb,
Islem Yangui,
Abdelkarim Aydi,
Imene Ouzari,
Hamdi Moktar
Affiliations
Taroub Bouzaiene
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), University of Carthage, Tunis 1080, Tunisia
Mariem Mohamedhen Vall
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), University of Carthage, Tunis 1080, Tunisia
Manel Ziadi
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), University of Carthage, Tunis 1080, Tunisia
Ines Ben Rejeb
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), University of Carthage, Tunis 1080, Tunisia
Islem Yangui
Higher Institute of Agronomy of Chott-Mariem, University of Sousse, BP. 47, Chott Mariem, Sousse 4042, Tunisia
Abdelkarim Aydi
French School Victor Hugo, Gontardstraße 11, 60488 Frankfurt am Main, Germany
Imene Ouzari
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 2092, Tunisia
Hamdi Moktar
Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT), University of Carthage, Tunis 1080, Tunisia
Previously, the exopolysaccharides produced by Lactiplantibacillus plantarum C7 isolated from the broiler intestine have been shown to possess probiotic potential. In this study, we highlighted the capacity of this strain to produce exopolysaccharide (EPS) endowed with several biological activities. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging activities of EPS were found to be 30.4% and 68.165%, respectively, at 100 mg/mL. The ferrous reducing potential of EPS was measured to be 25.26%. Furthermore, EPS exhibited antibacterial effects against both Gram-negative and Gram-positive pathogens, including Escherichia coli ATCC 10536 and Bacillus cereus ATCC 11778, with inhibition zones of 22.5 ± 0.70 mm and 20 ± 1.41, respectively. On the other hand, the exopolysaccharide also exhibited excellent inhibitory activity against butyrylcholinesterase (BChE), achieving 70.99% inhibition at a concentration of 10 mg/mL. FTIR spectra were used to characterize functional groups in EPS. Our findings proved that EPS from Lactiplantibacillus plantarum C7 could be explored for various applications, particularly in the health and functional food sectors.
