Determination of bioanalytical parameters for the standardization of Abroma augusta

Abstract

Objective: To set the analytical parameters for simultaneous determination of different phytochemicals present in the Abroma augusta (A. augusta). Methods: Different phytochemical test including total phenol and total flavonoid content were carried out in the present investigation. Further, a rapid and simple high-performance thin-layer chromatographic (HPTLC) method was developed for the qualitative and quantitative analysis. Moreover antimicrobial study was also performed in the present investigation to know the presence of biological contamination. Results: Preliminary phytochemical analysis revealed the presence of glycoside, alkaloid, carbohydrate and steroid in the A. augusta. Total phenol and total flavonoid content were found to be 0.29% and 0.50% w/w respectively. All tested microorganism in the A. augusta extract were found to be under the limit. Chloroform: methanol: acetic acid: H2O (170:25:25:4) was used as a solvent system for the qualitative and quantitative analysis through HPTLC Method. HPTLC analysis revealed 1.87 % w/w of linoleic acid in the A. augusta extract. Conclusions: Results showed that the selected parameters in the present investigation would set the new standards for the qualitative and quantitative analysis of A. augusta.

Keywords

• Abroma augusta
• HPTLC
• Phytochemical analysis
• Standardization