Turkish Journal of Agriculture: Food Science and Technology (Dec 2019)
Some Software’s Used in DNA Sequencing Based Haplotype Analysis
Abstract
Determination of genetic resources and their diversity constitutes are the initial step of the breeding programs. Before starting to the selection of the desired properties, many molecular methods are used to determine the existing genetic potential and determine the genotypic values. Many molecular methods (RAPD, RFLP, AFLP, SSR and DNA sequencing etc.) based on PCR technology are used to identify populations, identify genes related to yield and resistance, and demonstrate phylogenetic relationships. Many software’s for the analysis of molecular datasets obtained from molecular methods have been developed and the field of bioinformatics was born. In this study, after DNA sequence analysis; it is aimed to give information about haplotype analysis by determining SNPs (Single Nucleotide Polymorphism's) in individuals of populations, calculation of the genetic distance among populations and within populations, and software and analysis used for phylogenetic tree drawing.
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