Protective effect of Globularia alypum leaves against deltamethrin-induced nephrotoxicity in rats and determination of its bioactive compounds using high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole–time-of-flight mass spectrometry

Anouar Feriani; María del Mar Contreras; Nassima Talhaoui; Ana María Gómez-Caravaca; Amani Taamalli; Antonio Segura-Carretero; Lakhdar Ghazouani; Abdelfatteh El Feki; Mohamed Salah Allagui

Journal of Functional Foods (May 2017)

Protective effect of Globularia alypum leaves against deltamethrin-induced nephrotoxicity in rats and determination of its bioactive compounds using high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole–time-of-flight mass spectrometry

Anouar Feriani,
María del Mar Contreras,
Nassima Talhaoui,
Ana María Gómez-Caravaca,
Amani Taamalli,
Antonio Segura-Carretero,
Lakhdar Ghazouani,
Abdelfatteh El Feki,
Mohamed Salah Allagui

Affiliations

Anouar Feriani: Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia; Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, 3018 Sfax, Tunisia; Corresponding author at: Dr. Feriani Anouar, Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia.
María del Mar Contreras: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, 18016 Granada, Spain
Nassima Talhaoui: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, 18016 Granada, Spain
Ana María Gómez-Caravaca: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, 18016 Granada, Spain
Amani Taamalli: Laboratory of Olive Biotechnology, Center of Biotechnology of Borj-Cédria, BP. 901, 2050 Hammam-Lif, Tunisia
Antonio Segura-Carretero: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, 18016 Granada, Spain
Lakhdar Ghazouani: Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia
Abdelfatteh El Feki: Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, 3018 Sfax, Tunisia
Mohamed Salah Allagui: Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, 3018 Sfax, Tunisia

Journal volume & issue: Vol. 32
pp. 139 – 148

Abstract

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This study was designed to investigate the protective effects of Globularia alypum leaves extract (GE) against the nephrotoxicity provoked by a chronic exposure to deltamethrin (DLM) in rats. High-performance liquid chromatography coupled with electrospray ionization tandem quadrupole–time-of-flight mass spectrometry (HPLC-DAD-ESI-QTOF-MS) analysis revealed the presence of thirty-nine compounds among them globularin was the major compound (9444 mg/100 g of dry matter). Results showed that DLM administration altered the renal functions. Whereas, GE treatment restored the increased plasma creatinine, urea and uric acid levels, reduced the elevated MDA and PC levels, significantly normalized the antioxidant enzyme activities. In addition, histopathologic examination confirmed the deltamethrin damage to the renal tissue and revealed renal connective tissue fibrosis and severe necrosis of the proximal tubules with haemorrhage, which was corrected by GE treatment. Therefore, it was speculated that GE presented a protective effects against deltamethrin induced nephrotoxicity through the action of its bioactive compounds.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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