Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling

Hsiang-Ping Lee; Shan-Chi Liu; Yu-Han Wang; Bo-Cheng Chen; Hsien-Te Chen; Te-Mao Li; Wei-Chien Huang; Chin-Jung Hsu; Yang-Chang Wu; Chih-Hsin Tang

Journal of Functional Foods (Nov 2021)

Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling

Hsiang-Ping Lee,
Shan-Chi Liu,
Yu-Han Wang,
Bo-Cheng Chen,
Hsien-Te Chen,
Te-Mao Li,
Wei-Chien Huang,
Chin-Jung Hsu,
Yang-Chang Wu,
Chih-Hsin Tang

Affiliations

Hsiang-Ping Lee: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Chinese Medicine, China Medical University Hospital, Taichung 404024, Taiwan
Shan-Chi Liu: Department of Medical Education and Research, China Medical University Beigang Hospital, Yunlin 65152, Taiwan
Yu-Han Wang: Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404007, Taiwan
Bo-Cheng Chen: Department of Pharmacology, School of Medicine, China Medical University, Taichung 406040, Taiwan
Hsien-Te Chen: Department of Sports Medicine, College of Health Care, China Medical University, Taichung 406040, Taiwan; Department of Orthopedic Surgery, China Medical University Hospital, Taichung 404332, Taiwan
Te-Mao Li: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
Wei-Chien Huang: Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404007, Taiwan; Drug Development Center, China Medical University, Taichung 40402, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 404007, Taiwan
Chin-Jung Hsu: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Orthopedic Surgery, China Medical University Hospital, Taichung 404332, Taiwan; Corresponding authors at: Department of Orthopedic Surgery, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (C.J. Hsu). Chinese Medicine Research and Development Center, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (Y.C. Wu). Department of Pharmacology, School of Medicine, China Medical University, No. 100, Sec. 1, Jingmao Rd., Beitun Dist., Taichung City 406040, Taiwan (C.H. Tang).
Yang-Chang Wu: Chinese Medicine Research and Development Center, China Medical University Hospital, China Medical University, Taichung 404332, Taiwan; Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Corresponding authors at: Department of Orthopedic Surgery, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (C.J. Hsu). Chinese Medicine Research and Development Center, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (Y.C. Wu). Department of Pharmacology, School of Medicine, China Medical University, No. 100, Sec. 1, Jingmao Rd., Beitun Dist., Taichung City 406040, Taiwan (C.H. Tang).
Chih-Hsin Tang: Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404007, Taiwan; Department of Pharmacology, School of Medicine, China Medical University, Taichung 406040, Taiwan; Department of Biotechnology, College of Health Science, Asia University, Taichung 413305, Taiwan; Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan; Corresponding authors at: Department of Orthopedic Surgery, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (C.J. Hsu). Chinese Medicine Research and Development Center, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404332, Taiwan (Y.C. Wu). Department of Pharmacology, School of Medicine, China Medical University, No. 100, Sec. 1, Jingmao Rd., Beitun Dist., Taichung City 406040, Taiwan (C.H. Tang).

Journal volume & issue: Vol. 86
p. 104712

Abstract

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Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocyte into the joint synovium. Vascular cell adhesion molecule 1 (VCAM-1) is a critical cell adhesion molecule that controls monocyte motility during OA progression. Cordycerebroside A, a cerebroside compound isolated from Cordyceps militaris, inhibits the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in synovial macrophages, but has not yet been investigated in OA. Gene Expression Omnibus (GEO) dataset analysis revealed higher levels of VCAM-1 and CD11b (a monocyte marker) in OA synovial tissue compared with normal healthy tissue. The same results were observed in anterior cruciate ligament transaction (ACLT)-induced OA in rats compared with normal healthy controls. Cordycerebroside A markedly suppressed VCAM-1 expression and monocyte adhesion in human OA synovial fibroblasts. The MEK, ERK and AP-1 signaling cascades regulated cordycerebroside A-induced inhibition of VCAM-1 production. Thus, cordycerebroside A is a promising agent for the treatment of OA.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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