Kvasný průmysl (Mar 2002)
Application of Gel-Permeation Chromatography for Determination of Organic Pollutants in Brewing Barley.
Abstract
A method was developed that enables, within one extraction step, to determine nine polycyclic aromatic hydrocarbons (PAH) - benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, indeno(1,2,3-cd)pyrene,dibenzo(a, i)pyrene, dibenzo(a,h)pyrene - and seven indicating congeners of polychlorinated biphenyls (PCB) - congeners of No. 28, 52, 101, 118, 138, 153, 180 - in vegetable matrixes, mainly in barley and malt. The assay process includes the description of extraction and purification of the acquired extract by means of the gel-permeation chromatography method with the use of gel on the basis of polymer of styrenedivinylbenzene (Bio-Beads SX-3).In this way an extract is obtained of sufficient purity for the determination of both PAH and PCB. The final assay of PAH was carried out by the method of high-efficient liquid chromatography and the assay of PCB by gas chromatography using the electron capture detector. All analytes can be set in microgram amounts with a relative authoritative deviation < 25 %. The study contains characteristics of the method for all stipulated substances and speciment chromatograms.A method was developed that enables, within one extraction step, to determine nine polycyclic aromatic hydrocarbons (PAH) - benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, indeno(1,2,3-cd)pyrene,dibenzo(a, i)pyrene, dibenzo(a,h)pyrene - and seven indicating congeners of polychlorinated biphenyls (PCB) - congeners of No. 28, 52, 101, 118, 138, 153, 180 - in vegetable matrixes, mainly in barley and malt. The assay process includes the description of extraction and purification of the acquired extract by means of the gel-permeation chromatography method with the use of gel on the basis of polymer of styrenedivinylbenzene (Bio-Beads SX-3).In this way an extract is obtained of sufficient purity for the determination of both PAH and PCB. The final assay of PAH was carried out by the method of high-efficient liquid chromatography and the assay of PCB by gas chromatography using the electron capture detector. All analytes can be set in microgram amounts with a relative authoritative deviat
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