Oat globulin peptides regulate antidiabetic drug targets and glucose transporters in Caco-2 cells

Feng Wang; Yanyan Zhang; Tongtong Yu; Jiatai He; Jin Cui; Jingnan Wang; Xiangning Cheng; Junfeng Fan

Journal of Functional Foods (Mar 2018)

Oat globulin peptides regulate antidiabetic drug targets and glucose transporters in Caco-2 cells

Feng Wang,
Yanyan Zhang,
Tongtong Yu,
Jiatai He,
Jin Cui,
Jingnan Wang,
Xiangning Cheng,
Junfeng Fan

Affiliations

Feng Wang: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
Yanyan Zhang: Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China
Tongtong Yu: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
Jiatai He: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
Jin Cui: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
Jingnan Wang: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
Xiangning Cheng: Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China
Junfeng Fan: Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China; Corresponding author at: Department of Food Science and Engineering, College of Bioscience and Biotechnology, Beijing Forestry University, P.O. 112, 35 Qinghua East Road, Haidian District, Beijing 100083, China.

Journal volume & issue: Vol. 42
pp. 12 – 20

Abstract

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This study clarified the effects of well-characterized oat globulin peptides on antidiabetic drug targets (dipeptidyl peptidase IV [DPP4], α-glucosidase, glucose transporters 2 and 5 [GLUT2, GLUT5]) in Caco-2 cells. Twenty-two peptides with over 8 residues were identified from the tryptic hydrolysates (OGb, MM < 3 kDa) of oat globulin using liquid chromatography-mass spectroscopy. Computational docking demonstrated that LQAFEPLR (−8.8 kcal/mol) and EFLLAGNNK (−9.1 kcal/mol) effectively inactivated DPP4 binding to its active sites with low interaction energy. OGb, LQAFEPLR and EFLLAGNNK potently inhibited DPP4 and α-glucosidase activity in vitro concentration-dependently. Additionally, OGb (IC50, 188.1 μg/mL) and LQAFEPLR (141.7 μM) concentration-dependently inhibited DPP4 activity in Caco-2 cells. Specifically, they significantly downregulated the expression of DPP4 protein in Caco-2 cells (p < .05). However, these oat peptides also upregulated the expressions of α-glucosidase, GLUT2 and GLUT5 proteins in the cells. Overall, oat globulin peptides have contrasting modulatory effects on these antidiabetic drug targets.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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