EVALUATION OF A PHAGE PROTEIN-BASED ASSAY FOR THE DETECTION OF ESCHERICHIA COLI O157

Abstract

Phage tail recombinant protein technology developed by VIDAS UP O157 was used to detect E. coli O157 from bovine raw milk and milk filters. Sensitivity and specificity of VIDAS UP O157 was identical to a m-PCR assay for verocytotoxigenic E. coli detection.

Keywords

• Escherichia coli O157, recombinant phage proteins, immunofluorescence assay, milk, milk filters