Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial

Nadine G. Rouphael; Lilin Lai; Sonia Tandon; Michele Paine McCullough; Yunchuan Kong; Sarah Kabbani; Muktha S. Natrajan; Yongxian Xu; Yerun Zhu; Dongli Wang; Jesse O’Shea; Amy Sherman; Tianwei Yu; Sebastien Henry; Devin McAllister; Daniel Stadlbauer; Surender Khurana; Hana Golding; Florian Krammer; Mark J. Mulligan; Mark R. Prausnitz

doi:10.1038/s41541-021-00353-0

npj Vaccines (Jul 2021)

Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial

Nadine G. Rouphael,
Lilin Lai,
Sonia Tandon,
Michele Paine McCullough,
Yunchuan Kong,
Sarah Kabbani,
Muktha S. Natrajan,
Yongxian Xu,
Yerun Zhu,
Dongli Wang,
Jesse O’Shea,
Amy Sherman,
Tianwei Yu,
Sebastien Henry,
Devin McAllister,
Daniel Stadlbauer,
Surender Khurana,
Hana Golding,
Florian Krammer,
Mark J. Mulligan,
Mark R. Prausnitz

Affiliations

Nadine G. Rouphael: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Lilin Lai: Emory Vaccine Center, Department of Pediatrics, School of Medicine, Emory University
Sonia Tandon: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Michele Paine McCullough: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Yunchuan Kong: Laney Graduate School, Emory University
Sarah Kabbani: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Muktha S. Natrajan: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Yongxian Xu: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Yerun Zhu: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Dongli Wang: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Jesse O’Shea: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Amy Sherman: Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, School of Medicine, Emory University
Tianwei Yu: Laney Graduate School, Emory University
Sebastien Henry: Micron Biomedical, Inc.
Devin McAllister: Micron Biomedical, Inc.
Daniel Stadlbauer: Department of Microbiology, Icahn School of Medicine at Mount Sinai
Surender Khurana: Division of Viral Products Center for Biologics Evaluation and Research, FDA
Hana Golding: Division of Viral Products Center for Biologics Evaluation and Research, FDA
Florian Krammer: Department of Microbiology, Icahn School of Medicine at Mount Sinai
Mark J. Mulligan: New York University Langone Medical Center, Alexandria Center for Life Sciences
Mark R. Prausnitz: Micron Biomedical, Inc.

DOI: https://doi.org/10.1038/s41541-021-00353-0
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 9

Abstract

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Abstract In a phase 1 randomized, single-center clinical trial, inactivated influenza virus vaccine delivered through dissolvable microneedle patches (MNPs) was found to be safe and immunogenic. Here, we compare the humoral and cellular immunologic responses in a subset of participants receiving influenza vaccination by MNP to the intramuscular (IM) route of administration. We collected serum, plasma, and peripheral blood mononuclear cells in 22 participants up to 180 days post-vaccination. Hemagglutination inhibition (HAI) titers and antibody avidity were similar after MNP and IM vaccination, even though MNP vaccination used a lower antigen dose. MNPs generated higher neuraminidase inhibition (NAI) titers for all three influenza virus vaccine strains tested and triggered a larger percentage of circulating T follicular helper cells (CD4 + CXCR5 + CXCR3 + ICOS + PD-1+) compared to the IM route. Our study indicates that inactivated influenza virus vaccination by MNP produces humoral and cellular immune response that are similar or greater than IM vaccination.

Published in npj Vaccines

ISSN: 2059-0105 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy; Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.nature.com/npjvaccines/

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