ANTIPROLIFERATIVE ACTIVITY OF FLAVONOIDS ISOLATED FROM ECHINOPS GRACILIS O. HOFFM
CASTRO F. L. WÉYÉPÉ,
WILLIAM F. FEUDJOU,
CAROLE A. AMBONO,
HERMIA N. IKOME,
MARIUS M. BALEMAKEN,
PIERRE J. ABDOU,
THÉODORA K. KOPA,
GABRIEL A. AGBOR,
ALEMBERT T. TCHINDA
Affiliations
CASTRO F. L. WÉYÉPÉ
Institute of Medical Research and Medicinal Centre for Research on Medicinal Plants and Traditional Medicine, Plants Studies, Laboratory of Pharmacology and Drugs Discovery, P.O. Box 13033, Yaoundé, Cameroon
WILLIAM F. FEUDJOU
Institute of Medical Research and Medicinal Plants Studies, Centre for Research on Medicinal Plants and Traditional Medicine, Laboratory of Phytochemistry, P.O. Box 13033, Yaoundé, Cameroon
CAROLE A. AMBONO
University of Douala, Faculty of Science, Department of Chemistry, PO Box 24157, Douala, Cameroon
HERMIA N. IKOME
Institute of Medical Research and Medicinal Plants Studies, Centre for Research on Medicinal Plants and Traditional Medicine, Laboratory of Phytochemistry, P.O. Box 13033, Yaoundé, Cameroon
MARIUS M. BALEMAKEN
University of Yaoundé I, Faculty of Science, Department of Organic Chemistry, Box 812 Yaoundé, Cameroon
PIERRE J. ABDOU
University of Ngaoundéré, Faculty of Science, Department of Chemistry, P.O. Box 454 Ngaoundéré, Cameroon
THÉODORA K. KOPA
Institute of Medical Research and Medicinal Plants Studies, Centre for Research on Medicinal Plants and Traditional Medicine, Laboratory of Phytochemistry, P.O. Box 13033, Yaoundé, Cameroon
GABRIEL A. AGBOR
Institute of Medical Research and Medicinal Centre for Research on Medicinal Plants and Traditional Medicine, Plants Studies, Laboratory of Pharmacology and Drugs Discovery, P.O. Box 13033, Yaoundé, Cameroon
ALEMBERT T. TCHINDA
Institute of Medical Research and Medicinal Plants Studies, Centre for Research on Medicinal Plants and Traditional Medicine, Laboratory of Phytochemistry, P.O. Box 13033, Yaoundé, Cameroon
Apigenin-7-O-(4′′-feruloyl)-β-D-glucopyranoside (1) were isolated from the methanol extract of aerial part of Echinops gracilis, together with apigenin-7-O-(4′′-trans-p-hydroxycinnamoyl)-β-D-glucopyranoside (2) and apigenin-7-O-β-D-glucopyranoside (3). Compound (1) previously displayed antioxidant and antibacterial activity. The present study aims at evaluating the antiproliferative potential of flavonoids, isolated from the aerial part of E. gracilis O. Hoffm. The effect of compounds (1), (2) and (3) on the viability of HeLa cells was determined by the method of 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at different concentrations. The ability of compounds to induce the cell death was evaluated by using acridine orange/ethidium bromide (AO/EtBr) staining. Compound (1) induced an effective change in the cell viability of HeLa cells with IC50 concentration value of 27.36 µgmL-1. Induction of cell death alteration in cell morphology and cancer cell population was observed in cells treated with compound (1), which makes it behave as a potent synergistic antiproliferative agent against HeLa cells.
