IEEE Journal of the Electron Devices Society (Jan 2019)

Integrating a Plastic Glucose Biosensor Based on Arrayed Screen-Printed Electrodes Utilizing Magnetic Beads with a Microfluidic Device

  • Jung-Chuan Chou,
  • Si-Hong Lin,
  • Po-Yu Kuo,
  • Chih-Hsien Lai,
  • Yu-Hsun Nien,
  • Tsu-Yang Lai,
  • Tzu-Yu Su

DOI
https://doi.org/10.1109/JEDS.2019.2953093
Journal volume & issue
Vol. 7
pp. 1151 – 1160

Abstract

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In this study, a novel potentiometric glucose biosensor based on magnetic beads-Nafion-glucose oxidase/graphene oxide/aluminium-doped zinc oxide (MBs-Nafion-GOx/GO/AZO) membranes on a PET substrate with arrayed screen-printed electrodes (SPEs) was proposed. The morphologies, elemental analysis, and surface roughnesses of the sensing membranes were characterized. The sensing parameters of the biosensor were investigated by using the voltage-time (V-T) measurement system, which measured sensitivity, linearity, anti-interference ability, reversibility, temperature effect, and lifetime. A microfluidic device using polydimethylsiloxane (PDMS) was integrated with the V-T measurement system to analyze the response characteristics. The results under static solution and microfluidic flow conditions were compared. The results showed that the average sensitivity and linearity of the biosensor depended on the microfluidic flow rate. The best average sensitivity of the glucose biosensor based on MBs-Nafion-GOx/GO/AZO membranes was 20.35 mV/mM at a flow rate of $15~ \mu \text{L}$ /min.

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