Underlying action mechanism of a novel antioxidant peptide derived from Allium tuberosum Rottler protein hydrolysates and its protective effects on hydrogen peroxide induced cell injury

Abstract

A novel antioxidant peptide from protein hydrolysate of Allium tuberosum Rottler was identified as Phe-Pro-Leu-Pro-Ser-Phe (FF-6). It could not only quench multiple radicals efficiently, but also promote cell survival by protecting against oxidative damage. The residue Leu played a vital role in radical-scavenging process and interaction with ABTS·+ radicals. When substituted residue Leu with Ala, the radical-scavenging capacity and cytoprotective effect of FF-6 were almost lost. Moreover, the peptide could increase the expression level of proteins PI3K, Akt, phospho-Akt, mTOR, phospho-mTOR, but not affect peroxiredoxin-6. According to these results, the potential mechanism responsible for the protection of oxidative damage and promotion of cell survival was taken place through the PI3K/Akt/mTOR signaling pathway. These findings could be helpful for better elucidating the antioxidant mechanism and shed light on synthetic of alternative antioxidants with more potent activity as nutraceuticals or pharmaceuticals.

Keywords

• Antioxidant peptide
• Allium tuberosum Rottler
• Free radicals
• Oxidative damage
• Akt/mTOR signaling pathway