Journal of Functional Foods (Nov 2024)
Enhancing intestinal tight junction assembly by gallic acid as a subcellular basis for the pharmacological effect of Ocimum sanctum L. flower aqueous extract
- Wanapas Wachiradejkul,
- Pichayapa Sukmak,
- Supisara Treveeravoot,
- Laphatrada Yurasakpong,
- Nutnicha Rangchaikul,
- Pimngeon Chatkul,
- Pitsinee Supapol,
- Apiwan Arinno,
- Natnicha Teansuk,
- Jakkapong Inchai,
- Sukpapohn Phummisutthigoon,
- Makha Phongjit,
- Autsadakorn Loungjan,
- Nattaphong Akrimajirachoote,
- Wanangkan Poolsri,
- Chanat Aonbangkhen,
- Rungtiwa Khumjiang,
- Chatchai Muanprasat,
- Chutima S. Vaddhanaphuti,
- Pawin Pongkorpsakol
Affiliations
- Wanapas Wachiradejkul
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand
- Pichayapa Sukmak
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand
- Supisara Treveeravoot
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand
- Laphatrada Yurasakpong
- Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; In Silico and Clinical Anatomy Research Group (iSCAN), Bangkok, Thailand
- Nutnicha Rangchaikul
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand
- Pimngeon Chatkul
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand
- Pitsinee Supapol
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand
- Apiwan Arinno
- Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand; Center of Excellence in Natural Products Chemistry (CENP), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
- Natnicha Teansuk
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand
- Jakkapong Inchai
- Innovative Research Unit of Epithelial Transport and Regulation (iETR), Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
- Sukpapohn Phummisutthigoon
- Division of Physiology, Department of Preclinical Sciences, Faculty of Medicine, Bangkokthonburi University, Bangkok, Thailand
- Makha Phongjit
- Department of Biomedical Sciences, Faculty of Allied Health Sciences, Burapha University, Chonburi, Thailand
- Autsadakorn Loungjan
- Department of Biomedical Sciences, Faculty of Allied Health Sciences, Burapha University, Chonburi, Thailand
- Nattaphong Akrimajirachoote
- Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
- Wanangkan Poolsri
- Faculty of Science, Chulabhorn Royal Academy, Bangkok, Thailand
- Chanat Aonbangkhen
- Center of Excellence in Natural Products Chemistry (CENP), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Pathumwan, Bangkok, Thailand
- Rungtiwa Khumjiang
- Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan, Thailand
- Chatchai Muanprasat
- Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan, Thailand
- Chutima S. Vaddhanaphuti
- Center of Excellence in Natural Products Chemistry (CENP), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
- Pawin Pongkorpsakol
- Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand; Laboratory of Epithelial Tight Junction Pathophysiology, Bangkok, Thailand; Corresponding author at: Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, Thailand.
- Journal volume & issue
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Vol. 122
p. 106519
Abstract
Intestinal tight junction disruption initiates pathogenesis of colitis and determines diseases progression. At present, there is no therapeutics that directly corrects intestinal tight junction disassembly. Here, we discovered that Ocimum sanctum L. flower aqueous extract (OSLF) and gallic acid, increased intestinal barrier function by suppressing tight junction-dependent leak pathway permeability after being disrupted by Ca2+ depletion method. Transepithelial electrical resistance (TER) measurement, western blot analysis, immunofluorescence staining, and molecular docking indicated that OSLF and gallic acid improved intestinal barrier function by inducing tight junction assembly and inhibiting leak pathway permeability in intestinal epithelial cell monolayers via CaMKK-β/AMPK/SIRT-1/ERK-dependent fashion. Therefore, Gallic acid represents a drug candidate for treating diseases associated impaired intestinal barrier function including colitis.
