Two turtle-derived tripeptides as ARE-luciferase inducers suppress H2O2-induced oxidative stress

Shilei Wang; Jianchang Jin; Yi Wu; Nuo Chen; Baiyi Chu; Wei Wang; Nan Wang

Journal of Functional Foods (Dec 2024)

Two turtle-derived tripeptides as ARE-luciferase inducers suppress H2O2-induced oxidative stress

Shilei Wang,
Jianchang Jin,
Yi Wu,
Nuo Chen,
Baiyi Chu,
Wei Wang,
Nan Wang

Affiliations

Shilei Wang: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, PR China
Jianchang Jin: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, PR China
Yi Wu: School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, 310023 Hangzhou, PR China
Nuo Chen: The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, PR China
Baiyi Chu: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, PR China
Wei Wang: School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, 310023 Hangzhou, PR China; Corresponding authors.
Nan Wang: College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, PR China; Corresponding authors.

Journal volume & issue: Vol. 123
p. 106606

Abstract

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Activation of the Antioxidant response element (ARE) pathway contributes to protecting cells against oxidative stress. In the previous study, Asn-Cys-Ala and Cys-Thr-Ala derived from soft-shelled turtles demonstrated potent ARE-luciferase inducer activity in the HepG2 cell model. In this study, the effects of Asn-Cys-Ala and Cys-Thr-Ala on the Nuclear factor (erythroid-derived 2)-like 2/ Kelch-like ECH-associated protein 1 (Nrf2/Keap1) system in HepG2 cells were studied. The results showed that Asn-Cys-Ala and Cys-Thr-Ala regulated the Nrf2/ARE pathway by stabilizing the Nrf2 levels, which was achieved by decreasing the levels of Keap1. The EC50 values for the cellular antioxidant activity of Asn-Cys-Ala and Cys-Thr-Ala were 81.53 and 58.60 μM, respectively. And the results also demonstrated that treatment with Asn-Cys-Ala and Cys-Thr-Ala could reduce the H2O2-induced changes in intracellular Reactive Oxygen Species (ROS), Malondialdehyde (MDA) levels, and antioxidant enzyme activity (Superoxide dismutase, SOD; Glutathione peroxidase, GSH-Px; Catalase, CAT) (p < 0.05).

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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