Nutritional profile and DNA damage protective activity of Ottelia acuminata, an endemic plant from southwestern China

Abstract

Ottelia acuminata (O. acuminata) is an edible and medicinal submerged plant endemic to southwestern China, yet there is little information available concerning its nutritional or bio-active composition. This study aimed to evaluate in detail the nutritional profile and phenolic composition of O. acuminata (inflorescence, peduncle, and leaf) and investigate a protective activity in phenolic extracts on DNA damage induced by •OH and ROO• radicals. O. acuminata was excellent sources of protein (17.7–24 g/100 g DW), TPC (11.43–19.00 mg GAE/g DW), ascorbic acid, and minerals (especially potassium, calcium, and iron). Interestingly, the protein contained a high amount of EAA with a ratio (EAA11/TAA) in the range of 47.79–50.93%, BCAA and MSG-like AA. The inflorescences and leaves of O. acuminata contained abundant UFA with a ratio of UFA/TFA more than 50%. Five phenolic compounds, with a predominance of luteolin, luteolin-7-O-glucoside and chlorogenic acid, were identified by HPLC. Phenolic extracts of O. acuminata displayed a significant protective effect on DNA damage, which effect was comparable to that of the positive control (trolox). It was concluded that O. acuminata is an excellent vegetable with a high nutritional value and a great potential as a natural antioxidant.

Keywords

• Nutritional profile
• Phenolic extracts
• Oxidative DNA damage
• O. acuminata