A novel angiotensin I-converting enzyme inhibitory peptide from Phascolosoma esculenta water-soluble protein hydrolysate

Lei Du; Ming Fang; Hongxi Wu; Jingli Xie; Yanling Wu; Peng Li; Daihui Zhang; Zhenhua Huang; Yuelan Xia; Li Zhou; Dongzhi Wei

Journal of Functional Foods (Jan 2013)

A novel angiotensin I-converting enzyme inhibitory peptide from Phascolosoma esculenta water-soluble protein hydrolysate

Lei Du,
Ming Fang,
Hongxi Wu,
Jingli Xie,
Yanling Wu,
Peng Li,
Daihui Zhang,
Zhenhua Huang,
Yuelan Xia,
Li Zhou,
Dongzhi Wei

Affiliations

Lei Du: Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Ming Fang: Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Hongxi Wu: Zhejiang Key Lab of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, PR China
Jingli Xie: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Corresponding author at: P.O. Box 283, East China University of Science and Technology, 130 # Meilong Rd., Shanghai 200237, PR China. Fax: +86 21 64252563.
Yanling Wu: Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Peng Li: Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Daihui Zhang: Department of Food Science and Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Zhenhua Huang: Zhejiang Key Lab of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, PR China
Yuelan Xia: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Li Zhou: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China
Dongzhi Wei: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China

Journal volume & issue: Vol. 5, no. 1
pp. 475 – 483

Abstract

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The water-soluble protein from Phascolosoma esculenta was hydrolyzed by pepsin to obtain the hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity. The hydrolysate (PEPH) was then further separated by membrane bioreactor system, ion-exchange chromatography, gel filtration, and reversed phase high-performance liquid chromatography (RP-HPLC) and a novel ACE inhibitory peptide named as PeP with the IC50 value of 135 M was isolated. The amino acid sequence, Ala-Trp-Leu-His-Pro-Gly-Ala-Pro-Lys-Val-Phe, was identified by matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-TOF/TOF). Inhibitory kinetics study suggested that PeP acted as competitive inhibitor against ACE. Single oral administration of synthesized PeP at 10 mg/kg dose in spontaneously hypertensive rats could reduce the systolic blood pressure around 30 mmHg and the effect could last for more than 8 h. The results suggest that peptide from P. esculenta could be a potent natural ingredient for functional foods or pharmaceuticals against hypertension.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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