Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase

Giulia Di Persio; Manuela Pintore; Daniele Tonnina; Nicola Caretto; Elisabetta Martini; Dalina Lelo; Luigi Campanella

Food Technology and Biotechnology (Jan 2009)

Determination of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) in Milk and Fresh Cheese Based on the Inhibition of Cyclooxygenase

Giulia Di Persio,
Manuela Pintore,
Daniele Tonnina,
Nicola Caretto,
Elisabetta Martini,
Dalina Lelo,
Luigi Campanella

Affiliations

Giulia Di Persio: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Manuela Pintore: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Daniele Tonnina: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Nicola Caretto: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Elisabetta Martini: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Dalina Lelo: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy
Luigi Campanella: Department of Chemistry, University of Rome 'La Sapienza', P.le Aldo Moro 5, IT-00185 Rome, Italy

Journal volume & issue: Vol. 47, no. 2
pp. 172 – 177

Abstract

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A biosensor for rapid determination of nonsteroidal anti-inflammatory drugs (NSAIDs) is described based on the inhibition of cyclooxygenase enzyme (both isoforms) by NSAIDs. The results show the full validity of the method, which has also been optimized by comparing the inhibition of two enzyme isoforms, COX-1 and COX-2, in the presence of different tested pharmaceutical drugs (diclofenac, naproxen, ibuprofen, tolmetin). Also, recovery trials were performed in milk and fresh cheese adulterated with known quantities of NSAIDs, always obtaining recovery values >88 %.

Published in Food Technology and Biotechnology

ISSN: 1330-9862 (Print); 1334-2606 (Online)
Publisher: University of Zagreb Faculty of Food Technology and Biotechnology
Country of publisher: Croatia
LCC subjects: Technology: Chemical technology: Biotechnology; Technology: Chemical technology: Food processing and manufacture
Website: https://www.ftb.com.hr/

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