Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall.

Abstract

In order to optimize the extraction conditions of anthocyanins from Rosa davurica Pall. and investigate its antioxidant activity, single-factor experiment, Placket-Bumrman design, Box-Behnken analysis associated with ultrasonic extraction method were adopted. The antioxidant activity of anthocyanins from Rosa davurica Pall. were investigated by various methods, such as removal DPPH radical, \begin{document}${\rm{O}}_2^-\cdot $\end{document} radical, ABTS+ racial. The results showed that the optimum extraction conditions were as follows: Solid-liquid ratio was 1:15 g·mL−1, ultrasonic temperature was 50 ℃, ethanol volume was 60%, ultrasonic time was 30 min, pH was 2, and extraction times was 2. The content anthocyanins of Rosa davurica Pall. was 185.033 mg·100 g−1. The antioxidant activity was measured with IC50 and the results showed that the removal ability to DPPH free radical, superoxide anion free radical, ABTS+ free racial of the anthocyanins was 2.299, 1.902, 8.993 mg·mL−1, respectively. The technology in the current study was easy and feasible. The content of anthocyanins was considerable. Furthermore, it showed that the anthocyanins had certain antioxidant activity in various antioxidant experiments.

Keywords

• rosa davurica pall.
• anthocyanins
• plackett-burman design
• response surface analysis
• antioxidant activity