Optimization of canthaxanthin extraction from fermented biomass of Paracoccus carotinifacuens VTP20181 bacteria strain isolated in Vietnam

Abstract

Introduction. The bacterium strain Paracoccus carotinifaciens VTP20181 isolated in Vietnam produces canthaxanthin, a carotenoid widely used in the food and pharmaceutical industries. The aim of this work was to determine optimal parameters for canthaxanthin extraction from fermented biomass of P. carotinifaciens VTP20181. Study objects and methods. First, a series of single factor investigations were carried out in regard to maximal carotenoid content in the biomass extract obtained by using ultrasonic waves. Four parameters of the extraction process, such as extraction temperature, solvent/material ratio, extraction time, and ultrasonic output power, were studied. The obtained results were then optimized by using Response Surface Methodology (RSM) and Box-Behnken experimental design. Results and discussion. The optimal technological parameters of the extraction process included extraction temperature of 35°C, solvent/material ratio of 9.5:1 (v/w), extraction time of 90 min, and ultrasonic output power of 145 W. Under optimal conditions, canthaxanthin and total carotenoid contents were determined as 14.95 ± 0.12 and 18.21 ± 0.11 mg/g respectively, which were compatible with theoretical calculations ‒ 15.074 and 18.263 mg/g, respectively. Conclusion. Current results confirmed that the strain of halophilic P. carotinifaciens VTP20181 is a potential source for canthaxanthin biosynthesis.

Keywords

• paracoccus carotinifaciens vtp20181
• canthaxanthin
• total carotenoid
• optimization
• response surface methodology