Enhanced Fermentation Process for Production of High Docosahexaenoic Acid Content by <i>Schizochytrium</i> sp. GCD2032
Liucheng Long,
Xiaoqing Ren,
Feiyu Zhang,
Aijia Shi,
Yida Zhai,
Wuxi Chen,
Yu Duan,
Pengbao Shi,
Limei Chen,
Demao Li
Affiliations
Liucheng Long
College of Food Science & Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China
Xiaoqing Ren
College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China
Feiyu Zhang
College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300392, China
Aijia Shi
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Yida Zhai
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Wuxi Chen
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Yu Duan
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Pengbao Shi
College of Food Science & Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China
Limei Chen
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Demao Li
Tianjin Key Laboratory for Industrial Biological System and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
There is significant demand for high-purity DHA in the pharmaceutical industry. Traditionally, this high-purity DHA is extracted from raw materials with relatively low DHA content (10–20%), such as fish oil. Recently, through electroporation-induced mutation, a high-DHA-content strain of Schizochytrium sp. GCD2032 was isolated. To further enhance its DHA production, optimizations were conducted on the culture medium and fermentation conditions (in shaking flasks), as well as different nitrogen source concentrations (in a 5 L fermenter) for biomass, fatty acid content, and DHA content (as a percentage of total fatty acids). In a 5 L fermenter, Schizochytrium sp. GCD2032 achieved a biomass of 50 g/L, with fatty acid content of 55.71% and DHA content of 61.29%. Notably, the DHA content reached an impressive 341.45 mg/g of dry weight. This strain consistently produces high levels of fatty acids and DHA, demonstrating significant potential for pharmaceutical applications.
