Protective effects of LPL-EPS-02 on human dermal fibroblasts damaged by UVA radiation

Abstract

Long-term exposure of human skin to ultraviolet light can cause cell damage, and UVA mainly causes skin photoaging. The purpose of this study is to prove that the extracellular polysaccharide (LPL-EPS-02) isolated from a kind of Lactobacillus plantarum isolated from kimchi can protect and repair human fibroblasts after UVA irradiation. In this experiment, real-time reverse transcription-polymerase chain reaction (qRT-PCR) and ELISA methods were used to establish a UVA-irradiated human dermal fibroblast (HDF) model to explore the damage caused by UVA and LPL-EPS-02 protection and repair of the HDF from the cellular and molecular levels. LPL-EPS-02 can alleviate the oxidative stress caused by UVA irradiation, promote the production of antioxidant enzymes, and remove excessive free radicals. In addition, LPL-EPS-02 can also slow down the degree of collagen cross-linking caused by UVA and improve skin photoaging caused by sunlight. LPL-EPS-02 can also effectively prevent and repair skin photoaging caused by ultraviolet radiation by affecting the expression levels of TGF-β related transcription factors in HDF.

Keywords

• Lactobacillus plantarum polysaccharide
• Photoaging
• Damage repair