Identification of novel oligopeptides from the simulated digestion of sea cucumber (Stichopus japonicus) to alleviate Aβ aggregation progression

Xiaoling Lin; Maojin Yao; Jia-Hong Lu; Yi Wang; Xiquan Yin; Ming Liang; Erdong Yuan; Jiaoyan Ren

Journal of Functional Foods (Sep 2019)

Identification of novel oligopeptides from the simulated digestion of sea cucumber (Stichopus japonicus) to alleviate Aβ aggregation progression

Xiaoling Lin,
Maojin Yao,
Jia-Hong Lu,
Yi Wang,
Xiquan Yin,
Ming Liang,
Erdong Yuan,
Jiaoyan Ren

Affiliations

Xiaoling Lin: School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China
Maojin Yao: Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, China
Jia-Hong Lu: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
Yi Wang: State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of Hong Kong Polytechnic University, Shenzhen 518057, China
Xiquan Yin: Infinitus (China) Ltd., Guangzhou 510665, Guangdong, China
Ming Liang: Infinitus (China) Ltd., Guangzhou 510665, Guangdong, China
Erdong Yuan: School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China
Jiaoyan Ren: School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China; Sino-Singapore International Joint Research Institute, Guangzhou Knowledge City 510000, China; Corresponding author at: School of Food Science and Engineering, South China University of Technology, Wushan Rd. 381, Guangzhou 510641, China.

Journal volume & issue: Vol. 60

Abstract

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The aim of this study was to isolate and identify peptides from sea cucumber hydrolysates prepared by simulated gastrointestinal digestion, and to evaluate the selected peptides against amyloid beta (Aβ) aggregation using a mCherry-Aβ (E22G) cells model. The sub-fraction (F2-b-2) with the highest ORAC value (17.48 μmol TE/mg peptides) and the strongest anti-Aβ aggregation rate (36.12%), was purified by gel permeation chromatography and ion exchange chromatography. Nine novel oligopeptides (GMR, DVE, VFP, IGFH, LGFH, FQF, ICK, LCK and DWF) were identified from F2-b-2. By molecular docking, four of them (IGFH, LGFH, DWF and FQF) showed promising CD38 inhibitory activity. Besides, these four peptides (0.1 and 0.5 mM) also demonstrated significant anti-Aβ aggregation activity as compared to the model group (p < 0.01). This research suggested that oligopeptides derived from sea cucumber could be used as candidates for anti-Aβ aggregation, as well as the prevention or treatment of Alzheimer’s disease.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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