Hypouricemic effect of Grifola frondosa on hyperuricemic mice and virtual screening of bioactives by 3D QSAR pharmacophore modeling

Tianqiao Yong; Yizhen Xie; Shaodan Chen; Diling Chen; Jiyan Su; Chunwei Jiao; Huiping Hu; Chun Xiao

Journal of Functional Foods (Jan 2018)

Hypouricemic effect of Grifola frondosa on hyperuricemic mice and virtual screening of bioactives by 3D QSAR pharmacophore modeling

Tianqiao Yong,
Yizhen Xie,
Shaodan Chen,
Diling Chen,
Jiyan Su,
Chunwei Jiao,
Huiping Hu,
Chun Xiao

Affiliations

Tianqiao Yong: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China; Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510663, China; Corresponding author at: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China.
Yizhen Xie: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China; Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510663, China
Shaodan Chen: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China; Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510663, China
Diling Chen: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China
Jiyan Su: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China
Chunwei Jiao: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510663, China
Huiping Hu: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China
Chun Xiao: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China; Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510663, China

Journal volume & issue: Vol. 40
pp. 582 – 588

Abstract

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Grifola frondosa (GF) has been exploited as herb medicine for metabolic diseases and also as tasty food in Asia. Water (GFW) and ethanol (GFE) extracts produced remarkable hypouricemic effects, thereby decreasing the levels of serum uric acid from 240 μmol/L (hyperuricemia control, P < .01) to 170, 169, and 153 μmol/L for GFW at doses of 30, 60, and 120 mg/kg and to 169, 168, and 156 μmol/L for GFE at 50, 100, and 200 mg/kg correspondingly, which were similar to the normal control (159 μmol/L). ELISA analysis demonstrated the inhibitory effects of GFW and GFE on URAT1 protein rather than xanthine oxidase (XOD). The 3D-QSAR pharmacophore model for URAT1 was established successfully. Four compounds were identified and mapped adequately, deserving further study. Taken together, G. frondosa is a promising candidate for the development of functional foods.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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