Oxidative stability of fish oil dietary supplements and their cytotoxic effect on cultured human keratinocytes
Amanda Janaína Suzan,
Pedro Henrique Dias Garcia,
Cibele Priscila Busch Furlan,
Fátima Cristine Ribeiro Barba,
Yollanda Edwirges Moreira Franco,
Giovanna Barbarini Longato,
Fabiano Jares Contesini,
Patricia de Oliveira Carvalho
Affiliations
Amanda Janaína Suzan
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Pedro Henrique Dias Garcia
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Cibele Priscila Busch Furlan
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Fátima Cristine Ribeiro Barba
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Yollanda Edwirges Moreira Franco
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Giovanna Barbarini Longato
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil
Fabiano Jares Contesini
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
Patricia de Oliveira Carvalho
Health Sciences Postgraduate Program, São Francisco University, USF, Bragança Paulista, Brazil; Corresponding author at: São Francisco de Assis Avenue, 218, Cidade Universitária, Bragança Paulista, São Paulo, Brazil.
Commercially available fish oil supplements (FOS) are rich in n-3 polyunsaturated fatty acids (n-3 PUFAs) and have been prescribed as complementary therapy to reduce symptoms in many inflammatory skin diseases. The objective of this study was to assess the PUFAs content, oxidative stability and investigate the inhibitory effects on immortalized human keratinocytes (HaCaT) cell growth of FOS commercialized in Brazil. The fatty acids composition, analyzed by gas chromatography, was similar for all the capsules (comprising up to 30% n-3 PUFAs) and underwent no significant alteration during the storage period (9, 12, 18 and 24 months). Primary (peroxide), secondary (anisidine), and total oxidation products levels of only two FOS exceeded the maximum established by international quality standards, however, it is recommendable that the storage period should not exceed 18 months at the ambient temperature. Some products exhibited dose-dependent inhibitory effects on HaCaT proliferation.
