Application of SPE and SPME in Analysis of Beer.

Abstract

Solid phase extraction (SPE) and solid phase microextraction (SPME) are typical examples of modern sample preparation methods used more and more for the determination of flavour compounds in beer. The range of different sorbents for SPE and SPME fibers scales up. So the comparison of 11 SPE columns were investigated partly from the position of the same type of sorbents made by different producers and partly from different sort of sorbents. Furthermore the 3 type of SMPE fibers were compared. SPE sorbents and SPME fibers were tested by the extraction of fatty acids as an example.Working parameters of SPE and SPME methods were described in this study. The final determination of fatty acids were carried out using gas chromatograph Chrompack CP 9001 equipped with fused silica capillary column SPB-1000 (Supelco, length 30 m, i. d. 0,32 mm and 0,25 μm film thickness) and the flame ionisation detector.Solid phase extraction (SPE) and solid phase microextraction (SPME) are typical examples of modern sample preparation methods used more and more for the determination of flavour compounds in beer. The range of different sorbents for SPE and SPME fibers scales up. So the comparison of 11 SPE columns were investigated partly from the position of the same type of sorbents made by different producers and partly from different sort of sorbents. Furthermore the 3 type of SMPE fibers were compared. SPE sorbents and SPME fibers were tested by the extraction of fatty acids as an example.Working parameters of SPE and SPME methods were described in this study. The final determination of fatty acids were carried out using gas chromatograph Chrompack CP 9001 equipped with fused silica capillary column SPB-1000 (Supelco, length 30 m, i. d. 0,32 mm and 0,25 μm film thickness) and the flame ionisation detector.

Keywords

• solid phase extraction (SPE)
• solid phase microextraction (SPME)
• fatty acids
• beer