Synergistic effect of black tea polyphenol, theaflavin-3,3′-digallate with cisplatin against cisplatin resistant human ovarian cancer cells

Haibo Pan; Jin Li; Gary O. Rankin; Yon Rojanasakul; Youying Tu; Yi Charlie Chen

Journal of Functional Foods (Jul 2018)

Synergistic effect of black tea polyphenol, theaflavin-3,3′-digallate with cisplatin against cisplatin resistant human ovarian cancer cells

Haibo Pan,
Jin Li,
Gary O. Rankin,
Yon Rojanasakul,
Youying Tu,
Yi Charlie Chen

Affiliations

Haibo Pan: Department of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR China; College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV, USA
Jin Li: Department of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR China
Gary O. Rankin: Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USA
Yon Rojanasakul: Department of Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506, USA
Youying Tu: Department of Tea Science, Zhejiang University, Hangzhou, Zhejiang, PR China; Corresponding authors at: College of Science, Technology and Mathematics, Alderson Broaddus University, 101 College Hill Drive, Philippi, WV 26416, USA (Y.C. Chen). Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China (Y. Tu).
Yi Charlie Chen: College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV, USA; Corresponding authors at: College of Science, Technology and Mathematics, Alderson Broaddus University, 101 College Hill Drive, Philippi, WV 26416, USA (Y.C. Chen). Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, PR China (Y. Tu).

Journal volume & issue: Vol. 46
pp. 1 – 11

Abstract

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Theaflavin-3,3′-digallate (TF3) is a phenolic compound extracted from black tea. We previously demonstrated that TF3 selectively inhibited ovarian cancer cells. Ovarian cancer has high death rate because of acquired cisplatin resistance. We aimed to investigate the synergistic effect of TF3 and cisplatin (CDDP) against cisplatin resistant ovarian cancer cells. In the present study, combination treatment with TF3 and CDDP showed a synergistic cytotoxic effect in A2780/CP70 and OVCAR3 cells. Combination treatment showed a synergistic pro-apoptotic effect and synergistically induced G1/S phase cell cycle arrest. Synergistic apoptosis was accompanied by regulating protein expression of cleaved caspase 3/7, cytochrome c, Bax and Bcl-2. Combination treatment induced G1/S phase cell cycle arrest via regulating protein expression of cyclin A2, cyclin D1, cyclin E1 and CDK2/4. Combination treatment could synergistically down-regulate Akt phosphorylation in both cell lines. TF3 may be used as an adjuvant for the treatment of advanced ovarian cancer.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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