Evaluation of the anti-diabetic activity of sea buckthorn pulp oils prepared with different extraction methods in human islet EndoC-betaH1 cells
Eliza Korkus,
Grzegorz Dąbrowski,
Marcin Szustak,
Sylwester Czaplicki,
Rafał Madaj,
Arkadiusz Chworoś,
Maria Koziołkiewicz,
Iwona Konopka,
Edyta Gendaszewska-Darmach
Affiliations
Eliza Korkus
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Grzegorz Dąbrowski
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Marcin Szustak
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Sylwester Czaplicki
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Rafał Madaj
Division of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza, 112, 90-363 Lodz, Poland
Arkadiusz Chworoś
Division of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza, 112, 90-363 Lodz, Poland
Maria Koziołkiewicz
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Iwona Konopka
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Edyta Gendaszewska-Darmach
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland; Corresponding author.
Very uncommon within the plants, palmitoleic acid (POA) is present in high amount in the oil obtained from the pulp of the sea buckthorn (SB). Although POA was shown to exert anti-diabetic effects when used separately, more studies are needed to certify the application of POA present in SB oil. We show that the potency of SB pulp oil to augment glucose-induced insulin secretion has been associated with activation of G protein-coupled receptors present in pancreatic β-cells. The activity was shown in digested SB oils suggesting that released from triacylglycerols free fatty acid act as the active components. Among all fatty acids present in SB pulp oil POA demonstrated the highest activity. Analysis of five variants of SB oil samples prepared with different extraction methods revealed that the unsaponifiable compounds do not exert the synergic effect with the fatty acids.
