Cell wall component of Saccharomyces cerevisiae GILA 118 alleviates intestinal inflammation in DSS-induced colitis mice model

Abstract

In this study, Saccharomyces cerevisiae GILA 118 was used to separate cell wall components and protoplast to confirm the alleviation of inflammation using murine macrophage cells, RAW 264.7 cells, and C57BL/6J mice compared to the commercial probiotic strain Saccharomyces boulardii. In vitro and in vivo inflammation models were treated with S. cerevisiae GILA 118 cell wall polysaccharide and protoplast to verify the alleviating inflammation. Nitric oxide was reduced, cyclo-oxygenase-2 gene expression was reduced by peroxisome proliferator-activated receptor-γ expression in RAW 264.7 cells, and S. cerevisiae GILA 118 cell wall polysaccharide treated with zymolyase (PZ) significantly increased interleukin-10 compared to the dextran sulfate sodium mouse model. S. cerevisiae GILA 118 PZ induced Treg cell proportion and decreased Th 17/Treg cells proportion in the spleen. Thus, this study suggested that yeast cell wall components are potential alleviative agents in patients with inflammatory bowel disease.

Keywords

• Intestinal inflammation
• Saccharomyces cerevisiae
• Cell wall component
• Th17 cell
• Treg cell