FATTY ACIDS CONTENT AND ANTIBACTERIAL ACTIVITY OF Sargassum duplicatum LIPID

Abstract

Propionibacterium acne, Bacillus cereus and Escherichia coli are bacteria that cause infections of the skin and digestive tract in humans. Sargassum duplicatum, one of brown macroalgae species, is known to have the antibacterial activities. This study aims to prove antibacterial activities of S. duplicatum lipid against three different bacteria. The method used is the Folch method (using soxhletation, with a ratio of chloroform: methanol 2:1, v/v) to extract lipid. Furthermore, fatty acids content was identified by gas chromatography - mass spectrophotometry (GC-MS). Then, the disc diffusion method was used to measure the inhibition zone for antibacterial activity by using tetracycline as positive control. The lipid percentage of S. duplicatum was obtained about 2.502 % (v/w) where there were two lipid layers namely chloroform layer (0.388 % (v/w)) and methanol layer (2.114 % (v/w)). S. duplicatum lipid contained palmitic acid (in both layers) and oleic acid (only methanol layer). Moreover, methanol and chloroform lipid layer (1000 mg·mL-1) of S. duplicatum showed antibacterial activity against B. cereus, E. coli and P. acne

Keywords

• disc diffusion
• extraction
• folch method
• oleic acid
• palmitic acid