Effect of Compound Probiotic Powder of Lactobacillus rhamnosus and Bifidobacterium lactis on the Immune Function of Mice and Its Edible Safety

Abstract

In order to explore the effect of the compound probiotic powder of Lactobacillus rhamnosus and Bifidobacterium lactis on the immunity of mice and its food safety. In this study, low, medium and high doses (1.00, 0.67, 0.34g/kg BW) of compound probiotic powder were orally administered to mice to observe the organ/weight ratio, cellular immune function, humoral immune function, monocyte-macrophage function, NK cell activity and other indicators, comprehensively evaluate the effect of compound probiotic powder on the immune function of mice for 30 d. In addition, rat acute toxicity test, genetic toxicity test (mouse bone marrow cell micronucleus test, mouse sperm deformity test, Ames test) and 30-day feeding test for rats were used for food safety research. The results showed that, compared with the negative control group, the high-dose group mice had significantly increased delayed-type hypersensitivity (DTH), antibody cell production (PFC), serum hemolysin levels, and the phagocytosis index of carbon clearance in mice (P0.05). The growth and development of rats were good, and there was no significant difference in each index compared with the solvent control group (P>0.05). Therefore, the compound probiotic powder of Lactobacillus rhamnosus and Bifidobacterium lactis could enhance immunity without acute toxicity, genetic toxicity and subacute toxicity, and had high edible safety.

Keywords

• lactobacillus rhamnosus
• bifidobacterium lactis
• compound probiotic powder
• immunity
• edible safety