Antioxidant and hepatoprotective potential of sweet basil (Ocimum basilicum L.) extract in acetaminophen-induced hepatotoxicity in rats

Branislava Teofilović; Ana Tomas; Nikola Martić; Nebojša Stilinović; Milan Popović; Ivan Čapo; Nevena Grujić; Branislava Ilinčić; Aleksandar Rašković

Journal of Functional Foods (Dec 2021)

Antioxidant and hepatoprotective potential of sweet basil (Ocimum basilicum L.) extract in acetaminophen-induced hepatotoxicity in rats

Branislava Teofilović,
Ana Tomas,
Nikola Martić,
Nebojša Stilinović,
Milan Popović,
Ivan Čapo,
Nevena Grujić,
Branislava Ilinčić,
Aleksandar Rašković

Affiliations

Branislava Teofilović: University of Novi Sad, Faculty of Medicine, Department of Pharmacy, Hajduk Veljkova 3, 21000 Novi Sad, Serbia; Corresponding author.
Ana Tomas: University of Novi Sad, Faculty of Medicine, Department of Pharmacology, Toxicology and Clinical Pharmacology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Nikola Martić: University of Novi Sad, Faculty of Medicine, Department of Pharmacology, Toxicology and Clinical Pharmacology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Nebojša Stilinović: University of Novi Sad, Faculty of Medicine, Department of Pharmacology, Toxicology and Clinical Pharmacology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Milan Popović: University of Novi Sad, Faculty of Medicine, Department of Histology and Embryology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Ivan Čapo: University of Novi Sad, Faculty of Medicine, Department of Histology and Embryology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Nevena Grujić: University of Novi Sad, Faculty of Medicine, Department of Pharmacy, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Branislava Ilinčić: University of Novi Sad, Faculty of Medicine, Department of Pathophysiology and Laboratory Medicine, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Aleksandar Rašković: University of Novi Sad, Faculty of Medicine, Department of Pharmacology, Toxicology and Clinical Pharmacology, Hajduk Veljkova 3, 21000 Novi Sad, Serbia

Journal volume & issue: Vol. 87
p. 104783

Abstract

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This research aimed to examine the effects of pre-treatment with basil extract, prepared as an infusion, on acetaminophen-induced acute liver injury in rats. In vitro analysis of total phenolic, flavonoid content, and antioxidant activity was performed by spectrophotometric methods. Effects of basil extract on oxidative stress parameters, biochemical markers of liver injury, and liver morphology were determined in an in vivo model of acetaminophen-induced liver injury in Wistar rats. Total phenolic content was 52.61 ± 1.35 mg GAE/g of DE, and flavonoid content was 0.5 ± 0.2 mg QE/g of DE. IC50 values ranged from 0.22 to 45.76 μg/ml for DPPH radical, OH radical, H2O2 and lipid peroxidation. Ascorbic acid was used as a positive control. The extract lowered the intensity of lipid peroxidation and potentiated the activity of antioxidant enzymes. In addition, serum levels of aspartate aminotransferase and alanine aminotransferase were significantly lower in basil extract-treated animals in comparison to respective controls.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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