Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro

Xiaoyu Cai; Songxue Wang; Huali Wang; Suwen Liu; Guishan Liu; Huibin Chen; Ji Kang; Hao Wang

Food Science and Human Wellness (Jul 2023)

Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro

Xiaoyu Cai,
Songxue Wang,
Huali Wang,
Suwen Liu,
Guishan Liu,
Huibin Chen,
Ji Kang,
Hao Wang

Affiliations

Xiaoyu Cai: State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
Songxue Wang: State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
Huali Wang: China National Center for Food Safety Risk Assessment, Beijing 100022, China
Suwen Liu: College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
Guishan Liu: School of Food and Wine, Ningxia University, Yinchuan 750021, China
Huibin Chen: College of Life Sciences, Fujian Normal University, Fuzhou 350117, China
Ji Kang: State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; Correspondence authors at: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), No. 29, 13th Avenue, Economic and Technological Development Area (TEDA), Tianjin, China
Hao Wang: State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; Correspondence authors at: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), No. 29, 13th Avenue, Economic and Technological Development Area (TEDA), Tianjin, China

Journal volume & issue: Vol. 12, no. 4
pp. 1174 – 1183

Abstract

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The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells. In this study, SD rats were divided into the normal chow diet group (NCD), high fat diet group (HFD), three treatment groups feeding high-fat diet with naringenin (100, 200, 400 mg/kg) for 12 weeks. Results indicated that naringenin treatment decreased total cholesterol (TC), triglyceride (TG) and the non-high-density lipoprotein cholesterol (non-HDL-C) levels in serum. Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats. In addition, naringenin (25−75 µg/mL) decrease TG and TC levels in 3T3 mature adipocytes. The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology. Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes. Meanwhile, naringenin increased the AMPK (AMP-activated protein kinase) activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes. And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C. Molecular docking results indicated that naringenin could bind to AMPK protein. These results indicated naringenin reduced lipid accumulation through AMPK pathway.

Published in Food Science and Human Wellness

ISSN: 2213-4530 (Online)
Publisher: Tsinghua University Press
Country of publisher: China
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.sciopen.com/journal/2097-0765

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