Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment

Dong He; Wen Zeng; Yi Wang; Yifan Xing; Kang Xiong; Nan Su; Chong Zhang; Yuan Lu; Xinhui Xing

Food Science and Human Wellness (Nov 2022)

Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment

Dong He,
Wen Zeng,
Yi Wang,
Yifan Xing,
Kang Xiong,
Nan Su,
Chong Zhang,
Yuan Lu,
Xinhui Xing

Affiliations

Dong He: School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Wen Zeng: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Yi Wang: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Yifan Xing: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Kang Xiong: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Nan Su: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Chong Zhang: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Yuan Lu: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Xinhui Xing: Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China; Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 440300, China; Corresponding authors at: Tsinghua University, Beijing 100084, China.

Journal volume & issue: Vol. 11, no. 6
pp. 1464 – 1474

Abstract

Read online

Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF) were found to be effective in UC prevention in dextran sulfate sodium-induced mice in vivo. Herein, 4 novel peptides are identified from AC-WGAF by membrane ultrafiltration, recycling preparative high-performance liquid chromatography, and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight mass spectrometry, possessing anti-colitis activity via using an in vitro model. One of those peptides named T24 (PVLGPVRGPFPLL) exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss, maintaining epithelial barrier integrity, and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling pathways. Thus, T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment.

Published in Food Science and Human Wellness

ISSN: 2213-4530 (Online)
Publisher: Tsinghua University Press
Country of publisher: China
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.sciopen.com/journal/2097-0765

About the journal

Abstract

Keywords