Proteomics - a new and fast tool for identification of barley varieties.

Abstract

Identification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grainsIdentification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grains

Keywords

• proteomics
• protein identification
• barley
• variety identification
• mass spectrometry