Coumarin-rich Grifola frondosa ethanol extract alleviate lipid metabolism disorders and modulates intestinal flora compositions of high-fat diet rats

Abstract

This study investigated Grifola frondosa active substances extracted from low-concentration (55%) ethanol (GF55) and their role in regulating glucose and lipid metabolism disorders (GLMDs). The main components of GF55 were identified as fatty acids and coumarins by UPLC-Q-TOF-MS/MS After the intervention of GF55, the serum and liver lipid level, aspartate transaminase, alanine aminotransferase, free fatty acid, total superoxide dismutase, glutathione peroxidase, liver histopathological and key genes, short-chain fatty acids and intestinal flora changed. Compared with HFD, GF55 treatment up-regulated the expressions of protein kinase B, glucokinase, adenine monophosphate-activated protein kinase-α and cholesterol 7-α hydroxylase, down-regulated the expressions of stearoyl-CoA desaturase 1, acetyl-CoA carboxylase and sterol regulatory element-binding transcription factor-1c at the mRNA and protein levels (P < 0.01). Moreover, GF55 administration enriched the abundances of Alistipes, Paraprevotella and Ruminococcus2, whereas decreased the abundances of Romboutsia, Turicibacter and Coprococcus. GF55 might ameliorate GLMDs by modulating the functional composition of intestinal flora.

Keywords

• Grifola frondosa
• Coumarins
• Glucose and lipid metabolism
• Intestinal flora