Cichoric acid improves isoproterenol‐induced myocardial fibrosis via inhibition of HK1/NLRP3 inflammasome‐mediated signaling pathways by reducing oxidative stress, inflammation, and apoptosis

Abstract

Abstract Cichoric acid (CA), a natural phenolic compound found in many plants, has been reported to have antioxidant, anti‐inflammatory, hypoglycemic, and other effects. The aim of this study was to determine the potential role and underlying mechanisms of CA in isoproterenol (ISO)‐induced myocardial fibrosis (MF). The MF model was induced by subcutaneous ISO injection in mice. Blood and heart tissue were collected for examination. Hematoxylin and eosin staining and Masson's trichrome staining were used to evaluate the histopathological changes and collagen deposition. The production of reactive oxygen species markers was observed by fluorescence microscopy, the degree of cardiomyocyte microstructure injury was observed by transmission electron microscope, and oxidative stress factors were detected by kit method, and the effect of CA on inflammatory factors was detected by ELISA. The expression levels of collagen proteins and signaling pathways were further investigated by western blotting. The results showed that CA inhibited the expression of ISO‐induced proinflammatory factors (TNF‐α, IL‐1β, and IL‐18) and proteins (HK1, NLRP3, caspase‐1, cleaved‐caspase‐1, and ASC), and regulated the expression of apoptotic factors (caspase‐3, cleaved‐caspase‐3, Bax, and Bcl‐2). The results indicated that CA may regulate the HK1/NLRP3 inflammasome pathway by inhibiting HK1 expression and play a protective role in MF.

Keywords

• cichoric acid
• HK1/NLRP3
• myocardial fibrosis
• signaling pathway