Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches

Abstract

Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a need to discriminate pathogenic from non-pathogenic isolates rapidly and accurately. In this study, we have used standard culture based methods and advanced molecular approaches to characterize E. coli in food in a local outbreak investigation. We show that the application of DNA based detection methods including real-time PCR and DNA microarray along with a traditional culture method can identify the organism implicated in an outbreak at the strain level for pathogenic potential.

Keywords

• E. coli non-O157
• Shiga toxin producing E. coli (STEC)
• RT-PCR
• DNA microarray