International Journal Bioautomation (Apr 2015)

Constructing Optical Biosensor Based on Multienzyme System Tyrosinase/Horseradish Peroxidase

  • Lyubov Yotova,
  • Spaska Yaneva

Journal volume & issue
Vol. 19, no. suppl. 1
pp. S43 – S50


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By using sol-gel method, hybrid membranes were synthesized based on SiO2 and cellulose derivatives. Membranes showed good physical and mechanical properties as well as good biocompatibility. Onto hybrid membranes tyrosinase and horse radish peroxidase were immobilized and optical biosensor was constructed. The working principle of constructed biosensor was detection of oxygen consumption. The properties of constructed biosensors like linear range, life time and sensitivity were defined by modeling substrate - L-3,4-dihydroxyphenylalanine. The factors affecting the constructed optical biosensors were investigated. The results showed that a multienzyme system is capable of improving the constructed biosensor properties.