The dose-dependent pteryxin-mediated molecular mechanisms in suppressing adipogenesis in vitro

Ruwani N. Nugara; Hirosuke Oku; Seiko Saitoh; Masashi Inafuku; Hironori Iwasaki; R.P. Kumara; L.J.S. Undugoda; B.N.S. Lankasena

Journal of Functional Foods (Jul 2021)

The dose-dependent pteryxin-mediated molecular mechanisms in suppressing adipogenesis in vitro

Ruwani N. Nugara,
Hirosuke Oku,
Seiko Saitoh,
Masashi Inafuku,
Hironori Iwasaki,
R.P. Kumara,
L.J.S. Undugoda,
B.N.S. Lankasena

Affiliations

Ruwani N. Nugara: Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka; Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan; Corresponding author at: Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka.
Hirosuke Oku: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan
Seiko Saitoh: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan; College of Economy and Environmental Policy, Okinawa International University, 2-6-1 Ginowan, Okinawa 901-2701, Japan
Masashi Inafuku: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan; Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan
Hironori Iwasaki: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan
R.P. Kumara: Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa 903-0213, Japan
L.J.S. Undugoda: Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka
B.N.S. Lankasena: Department of Information and Communication Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka

Journal volume & issue: Vol. 82
p. 104508

Abstract

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The aim was to perform an in-depth characterization of pteryxin in the anti-obesity related molecular mechanisms using 3T3-L1 preadipocytes. Cells were treated with pteryxin 20 (PTX20) or 50 (PTX50) μg/mL during cell differentiation or maintenance period. Pteryxin dose-dependently suppressed adipogenic gene network. PTX20 downregulated key lipogenic genes suppressing fatty acid synthesis allotting the anti-adipogenesis in the late adipocyte formation stage. PTX50 downregulated adipogenesis related genes, and accelerated lipolytic activity by repressing the adipocyte size marker gene, MEST. In addition to Wnt5a, PTX50 suppressed adipocyte differentiation through TGF-β/Smad3 signaling pathway with the maximum adiposity inhibition in the early stage of adipogenesis and regulated the carbohydrate metabolism. Pteryxin regulated phosphorylation of ERK1/2 and AMPK, and decreased p38 MAPK to repress the mature adipocyte formation. Overall, we have demonstrated the anti-adipogenic characteristics of pteryxin consistent to its dose. PTX50 may serve as a potential candidate to ameliorate adiposity and improve the carbohydrate metabolism.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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