The insulinotropic activity of oleosomes prepared from various sea buckthorn cultivars in mouse and human pancreatic β cell lines
Eliza Korkus,
Marcin Szustak,
Grzegorz Dąbrowski,
Sylwester Czaplicki,
Sławomir Kadłubowski,
Maria Koziołkiewicz,
Iwona Konopka,
Edyta Gendaszewska-Darmach
Affiliations
Eliza Korkus
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Marcin Szustak
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Grzegorz Dąbrowski
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Sylwester Czaplicki
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Sławomir Kadłubowski
Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland
Maria Koziołkiewicz
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland
Iwona Konopka
Faculty of Food Sciences, Chair of Plant Food Chemistry and Processing, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957 Olsztyn, Poland
Edyta Gendaszewska-Darmach
Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland; Corresponding author.
Sea buckthorn berries contain numerous bioactive lipids such as fatty acids, carotenoids, tocols, and sterols. Among fatty acids, a high concentration of rare in nature palmitoleic acid is characteristic of sea buckthorn pulp oil. We previously showed the potency of pulp oil from the Luczystaja cultivar to augment glucose-induced insulin secretion in pancreatic β cells. Since the composition of sea buckthorn berries varies depending on the cultivar, here we compared the activity of oleosomes from Botaniczeskaja Ljubitelskaja, Golden Rain, Prozracznaja, Maryna and Luczystaja cultivars in two different pancreatic cell lines, a mouse MIN6 and human EndoC-betaH1. Besides the characterization of particle size and ζ-potential, we evaluated the influence of crude and digested oleosomes on cell viability, insulin secretion, and intracellular calcium mobilization. Additionally, the antioxidant activity measured with the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical cation-based assay was determined and compared.
