Shipin gongye ke-ji (Dec 2022)

Optimization of Enzymatic Hydrolysis Process of Pueraria Protein by Response Surface Methodology and Its Antioxidant Properties in Vitro

  • Huina PANG,
  • Hongying DONG,
  • Fengqin XIAO,
  • Hongyin ZHANG,
  • Rongxin HAN,
  • Haidong WANG,
  • Mingming YAN,
  • Shuai SHAO

DOI
https://doi.org/10.13386/j.issn1002-0306.2021100147
Journal volume & issue
Vol. 43, no. 24
pp. 197 – 204

Abstract

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Objective: To optimize the enzymatic hydrolysis process of Pueraria protein and study its antioxidant properties. Methods: DPPH radical scavenging ability and DH were used as the index to screen the best hydrolytic protease. Base on the single factor experiments, the Box-Behnken response surface design was used to optimize the enzymatic hydrolysis process of Pueraria protein, and the antioxidant properties of the optimum hydrolysate in vitro was analyzed. Results: The optimal hydrolysis conditions for alkaline protease were as follows: Temperature 55 ℃, pH9 and enzyme substrate ratio 2%. Under these conditions, the IC50 of DPPH·, ABTS+·, and ·OH radicals were 0.15, 0.38, 1.41 mg/mL and a reduction capacity of 0.553. Conclusion: Under these conditions, Pueraria protein hydrolysate had remarkable antioxidant properties.

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