Blueberry anthocyanins ameliorate cyclophosphamide-induced liver damage in rats by reducing inflammation and apoptosis

Lin Shi; Yun-en Liu; De-hong Tan; Ting-cai Yan; De-qun Song; Ming-xiao Hou; Xian-jun Meng

Journal of Functional Foods (Nov 2014)

Blueberry anthocyanins ameliorate cyclophosphamide-induced liver damage in rats by reducing inflammation and apoptosis

Lin Shi,
Yun-en Liu,
De-hong Tan,
Ting-cai Yan,
De-qun Song,
Ming-xiao Hou,
Xian-jun Meng

Affiliations

Lin Shi: Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, China; College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
Yun-en Liu: Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, China; Key Laboratory of Severe Trauma and Organs Protection in Liaoning, General Hospital of Shenyang Military Command, Shenyang 110016, China
De-hong Tan: College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
Ting-cai Yan: College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
De-qun Song: College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
Ming-xiao Hou: Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, China; Corresponding author. Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, China. Tel.: +86 24 28851293; fax: +86 24 28856615.
Xian-jun Meng: College of Food Science, Shenyang Agricultural University, Shenyang 110161, China; Corresponding author. Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, China. Tel.: +86 24 28851293; fax: +86 24 28856615.

Journal volume & issue: Vol. 11
pp. 71 – 81

Abstract

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We attempted to determine whether blueberry anthocyanins (BA) would be protective against cyclophosphamide (CTX)-induced liver injury in SD rats. CTX was injected intraperitoneally into rats to induce liver toxicity. The rats were randomly divided into four groups: a control group, a CTX group, and two groups received BA at doses of 20 and 80 mg/kg/day for 7 days (i.g.), both before and after they were administered CTX. Liver histopathology, serum liver enzymatic activities, cytokines and apoptotic parameters were evaluated. The rats that were injected with CTX incurred liver injury, evidenced by histological changes and elevated serum enzymes activities; CTX led to elevated proinflammatory cytokines, and reduced anti-inflammatory cytokine; also induced apoptosis, indicated by increased Bax and TLR4, and decreased Bcl-2 expression in western blot assay, which was further confirmed by immunohistochemistry assay with anti-Bax antibody. BA had a protective effect against CTX-induced liver damage in rats.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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