Harčova Nauka ì Tehnologìâ (Jun 2020)
INVESTIGATION OF THE STRUCTURE OF WATER-SOLUBLE GLUCAN YEAST SACCHAROMYCES CEREVISIAE
Abstract
It is known that a well-functioning immune system is important for human health. There are many natural and synthetic preparation that are widely used as immunomodulators. One such natural preparat is β-glucan. Beta-glucans are a group of natural polysaccharides. They are recognized as an effective immunocorrector. Their use is advisable both for the prevention of immunodeficiency pathologies and for the complex treatment of many diseases from cardiovascular to oncological. The physiological activity of β-glucan depends on the type and configuration between monosaccharide residues, branching and conformation of macromolecules, solubility in water. One major source of β-glucan is the baker’s yeast Saccharomyces cerevisiae. Much research has been carried out over the years examining cell wall glucans from Saccharomyces cerevisiae. This work is the development devoted to the characterization of water-soluble beta-glucan obtained as a result of controlled degradation with the enzyme Rovabio Excel AP of glucan cell walls of yeast Saccharomyces cerevisiae. In this study conditions were selected which allow to accumulate the maximum water-soluble fractions with a molecular mass of 1–30 kDa presumably as fractions with a high immunomodulatory effect. The results of the paper show that glucan can be isolated from Saccharomyces cerevisiae in very pure form by the method used in this study. Thus structural analysis gives reliable results. The structural characterization of pure product was performed using the common analytical procedures: enzymes hudrolyses and spectral analyses FTIR, NMR spectroscopy. On the basis of the obtained results it was concluded that investigated glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains with sparsely branched. Further work will concern the physiological effect of water-soluble glucan in comparision to the native glucan. The structural requirements for example for an immunomodulation in humans or animals are still under discussion.
Keywords
