Effect of Unsaturated Fatty Acid Ratio In Vitro on Rumen Fermentation, Methane Concentration, and Microbial Profile
Zhantao Yang,
Siyuan Liu,
Tian Xie,
Qianqian Wang,
Zhonghan Wang,
Hongjian Yang,
Shengli Li,
Wei Wang
Affiliations
Zhantao Yang
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Siyuan Liu
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Tian Xie
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Qianqian Wang
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Zhonghan Wang
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Hongjian Yang
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Shengli Li
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Wei Wang
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
It is well known that dairy cows are fed diets with high fat content, which can adversely affect rumen fermentation. However, whether the effects of high fat content on rumen fermentation are related to the composition of fatty acids (FA) is for further study. We explored the effects of unsaturated fatty acid (UFA) ratios in vitro on rumen, methane concentration and microbial composition under the same fat levels. The experiment included a low-unsaturated group (LU, UFA proportion: 42.8%), a medium-unsaturated group (MU, UFA proportion: 56.9%), and a high-unsaturated group (HU, UFA proportion: 70.9%). The incubation fluid pH and NH3-N levels were not significantly different in the three groups. Total volatile fatty acid (TVFA), acetate, propionate, butyrate, and valerate in the MU group had a decreased trend compared to the LU group (0.05 p 4 concentration was significantly higher than the HU group (p 2 concentration in the LU group was also significantly higher than the MU and LU groups (p p Succinivibrionaceae_UCG_001 and Fibrobacter (p Lachnospiraceae bacteria significantly increased in the MU group (p 4 concentration was reduced.
